Contact: Dr. Carmen Rubio, Molecular Cytogenetics and PGD
KARYOLITE is a simple, rapid, reproducible technique with an optimised cost/result ratio. It detects the aneuploidies of the 23 pairs of chromosomes.
This technique is done using probes bound to polystyrene microspheres which are recognised by the Luminex reader by quantifying the amount of fluorescence and comparing it with a normal pattern.
This is the molecular version of the conventional karyotype in which chromosomes can be seen under the microscope. It commences when the conventional karyotype cannot be done as a result of cell culture problems. The result is obtained in 10-15 days after receiving the sample. It proves most useful in the study of aneuploidies in abortive remains in which the sample does not grow after the cell culture given its conditions.